YMC*Gel PolymerC18

based on hydrophilic polymethacrylate support for a performance similar to silica-based C18 for greater reproducibility methacrylate is functionalised with C18 before polymerisation no silanols or metal contaminants pH stable from pH 2.0 - 13.0 compatible with all standard reversed phase solvents

General

YMC*Gel PolymerC18 is a pure organic packing which provides a broad range of solvent choices and a pH range from 2 – 13. It is manufactured from a hydrophilic methacrylate polymer which is cross-linked with C18 ligand-containing reagents. YMC*Gel PolymerC18 offers a maximum application range: A wide variety of compounds such as organic acids, organic amines, peptides, pharmaceuticals and proteins can be separated using YMC*Gel PolymerC18.

Properties

YMC*Gel PolymerC18 is prepared from a hydrophilic methacrylate polymer bonded with a hydrophobic octadecylsilane reagent to make the C18 functionality an integral part of the polymeric structure. As Polymer C18 is a three-dimensional polymer matrix which is not based on a silica gel support, it has no residual silanols or metal impurities to interfere with the separation of basic organic compounds.

The selectivity and retention of YMC*Gel PolymerC18 is similar to standard ODS phases, due to its hydrophobic bonding on a hydrophilic support. Consequently, its selectivity is closer to that of silica-based C18 supports than to styrene/DVB-based supports. It should be noted that interactions between aromatic or conjugated systems and the p-electrons of the carbonyle group of the methacrylate backbone provide slightly greater retention when compared to silica-based ODS columns, whereas highly aliphatic compounds show greater retention on silica-based ODS supports.

YMC*Gel PolymerC18 is compatible with all common reversed phase eluents such as water, methanol, acetonitrile and THF. Virtually all aqueous buffers and acid modifiers, such as TFA and phosphoric acid, as well as base modifiers such as sodium hydroxide and ammonium hydroxide can be used. Since it resists shrinking and swelling, YMC*Gel PolymerC18 can be used with eluents ranging in composition from 100% aqueous to 100% organic component.

In addition, YMC*Gel PolymerC18 can easily be sterilised by flushing with 0.1N NaOH in 20 % acetonitrile/water.