Preparative LC : Bulk Packing Material : YMC Gel Diol

Bulk Packing Material

YMC*Gel Diol

For Normal Phase applications:

For aqueous GPC/SEC applications:

versatile alternative to silica
bonded phase reproducibility
good selectivity without
excessive retention
high preparative throughput
for polar natural products, metabolites, lipids

high mechanical stability
for molecular weight determination of proteins, peptides and sugars

YMC*Gel Diol	Specification
Pore size / nm	6	12	20	30
Particle size / µm	5	5; 10; 15	5; 15	5
Surface area / m2g-1	650	330	175	100
Carbon content / %	-	-	-	-
Recommended pH range	2.0 - 7.5	2.0 - 7.5	2.0 - 7.5	2.0 - 7.5
Note: Analytical grades (5 µm) are not available as bulk materials; they are only available in prepacked columns.

A normal phase alternative to silica

YMC*Gel Diol is a versatile alternative to silica for normal phase separations. The bonded phase’s hydroxyl groups provide good selectivity without excessive retention, because hydrogen bonding with the diol layer is not as strong as with the silanols on a bare silica surface. Diol columns also provide improved reproducibility when compared with bare silica.

As with all YMC silica based bonded phases, YMC*Gel Diol starts with a base silica support of exceptional purity and mechanical stability. The silica purity greatly reduces non-specific sample adsorption, thereby providing excellent sample recovery. The high surface area, together with the large number of available sites for interaction of the 1,2-dihydroxypropane ligands, provides high preparative loading.

YMC*Gel Diol can be cleaned repeatedly with methanol or even water, thereby ensuring a longer column life than possible with underivatised silica.

Silica based aqueous GPC/SEC medium

The silica based YMC*Gel Diol is a rugged and efficient size exclusion material. It is generally used for the separation of biomolecules and pharmaceuticals. As the non-specific adsorptive sites have been eliminated it exhibits better performance characteristics for size separations than underivatised silica. YMC*Gel Diol is available in four porosities: 6, 12, 20 and 30 nm. Diol-6 is most suitable for the separation of peptides or oligosaccharides with molecular weights of 10,000 or less, whereas Diol-12, -20 and -30 (either used individually or in combination) are suitable for separations of proteins and other water-soluble compounds with molecular weights of 10,000 to several hundred thousand dalton.