YMC*Gel C4 (Butyl)

low hydrophobicity phase high coverage monomeric bonding fully endcapped ideally suited for the separation of biomolecules, especially non-polar peptides and proteins

General

Due to shorter alkyl chains YMC*Gel C4 has a lower hydrophobicity than both C18 and C8 phases. Therefore retention times of non-polar samples tend to be shorter on YMC*Gel C4, making it an ideal choice for faster separations.

Properties

YMC*Gel C4 packings are less hydrophobic and generally require more aqueous conditions than C8 or C18 packings. When using the same eluent, YMC*Gel C4 shows significantly shorter retention times for non-polar compounds than either C8 or C18 phases. Retention of polar compounds, however, is not considerably affected. Therefore, YMC*Gel C4 is ideally suited for separating complex samples with a wide range of component polarity: The butyl bonded phase gives shorter retention times while still maintaining high resolution when compared to longer chain bonded chemistries as demonstrated in the figure below.

YMC*Gel C4 is available in three porosities to allow optimum separations of virtually any molecular weight compound. The 12 nm phase is used extensively with non-polar peptides which show very long retention times on C8 and C18. The 20 nm and 30 nm pore butyl phase packings effectively resolve many classes of proteins and biomolecules. The wide pore butyl chemistry allows minimal distortion of the tertiary conformation of large biomolecules and results in fractions that are pure, concentrated and retain high biological activity.