Separation of human serum
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| Column |
YMC-BioPro Q75, 50 x 4.6 mm I.D. |
| Eluent |
A) 20 mM Tris-HCI (pH 8.6)
B) 1.0 M NaCl in 20 mM Tris-HCI (pH 8.6) |
| Gradient |
0% B (0-3 min), 0-15% B (3-18 min), 15-50% B (18-33 min), 50% B (33-40 min) |
| Flow rate |
0.5 mL/min (180 cm/hr) |
| Temperature |
25 °C |
| Detection |
UV at 280 nm |
| Injection |
20 mL (110 µL/mL) |
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