Products : General : Stationary Phase

Products

General : Stationary Phases

YMC has built its reputation on the supply of consistent, premium quality HPLC media and columns. YMC is one of the few suppliers of chromatography media that synthesises its own spherical silica supports in the manufacture of its chromatography media. Control of the silica support is essential for product consistency and is of the highest priority to YMC. The silica supports are then functionalised with the Company’s proprietary bonding and endcapping chemistries to yield rugged, reproducible, high performance HPLC packings.

Stationary phases routinely available from YMC

An overview of analytical stationary phases routinely available from YMC can be found in our Download-section.

YMC Phase Selection Guide

The Importance of Selectivity: The basis for every successful separation is the choosing of the appropriate selectivity. YMC provides an extensive selection of more than 30 chromatographic phases to address virtually any separation need. Many of these stationary phases are fully scalable and often available with a wide range of pore sizes from 6 to 100 nm and particle sizes from 3 to 150 µm. In addition to that, most YMC products can be further customised with regards to pore size, endcapping and/or carbon content to increase adaptability to a specific customer’s separation. (The "YMC Phase Selection Guide" (pdf-document) can be found here).

* YMC-Pack ODS-AQ and Hydrosphere C18 shows increased interaction with polar compounds at highly aqueous mobile phases.

Hydrophobicity

Characterises the strength of hydrophobic interaction and surface coverage of bonding measured by the retention factor of a purely hydrophobic analyte (e.g. k’ Acenaphthene).

Select packings further to
the right to increase retention
under a given mobile phase.

Silanol Activity

Indicates the presence of residual silanol groups at silica surface and/or the capability of hydrogen bonding due to special surface chemistry (see ODS-AQ and Hydrosphere C18).

Measured by using the relative retention between an analyte that interacts by hydrophobic and silanophilic interaction (e.g. Amitriptyline) and an analyte that interacts by hydrophobic interaction only (e.g. Acenaphthene).

Low silanol activity is shown by high purity silicas (lower part of the chart) and is recommended for the analysis of basic compounds; high silanol activity is shown by lower purity silicas which are often not endcapped.

Choose your selectivity

Basic compounds or LC/MS analysis: choose packings in the lower part of the chart.

Differences in hydrophobicity: choose packings that are far away from each other on the x-axis.

Differences in polar selectivity:
select packings that are located in different sections of the chart.

Columns close to each other will have similar selectivity but be aware that small differences are possible.

Additional information can be found in sections "Analytical Columns" and "Preparative LC : Bulk Packing Materials".