 |
| Column: |
5 μm, 50 x 2.0 mm ID |
| Eluent: |
A) water / fomic acid (100/0.1)
B) acetonitrile /formic acid (100/0.1)
5% B (0-1 min), 5-100% B
(1-5 min), 100% B (5-10 min),
100-5% B (10-10.1 min),
5% B (10.1-12.5 min) |
| Flow rate: |
0.4 ml/min |
| Temperature: |
40 °C |
| Detection: |
ESI positive, TIC (Mass Range: 50-1000) |
|
| Column bleeding, caused by the fragments of stationary phase, is the main reason for background noise and limitations on detection limits. No bleed is observed in the test of total ion current (TIC) measured by LC/MS with blank or with YMC-Triart C18. So in terms of the signalnoise ratio (S/N ratio), YMC-Triart C18 can be expect to not only reduce the background noise but to also increase the sensitivity of the analysis. |
| Column: |
TA12SP90502PT |
| Dimension: |
50 x 2.0 mm ID |
| Eluent: |
A) 20 mM NH4CH3COOH + ammonia (pH 9.0)
B) acetonitrile |
| Gradient: |
25% B (1.0 min); 75% B (1-6 min) |
| Flow rate: |
0.35 ml/min |
| Detection: |
UV at 254 nm |
| Pressure: |
450 bar |
| Temperature: |
40 °C |
| Injection: |
1 μl |
| Column: |
YMC-TriArt C18 (5 μm, 12 nm) |
| Dimension: |
50 x 2.0 mm ID |
| Eluent: |
A) 20 mM HCOONH4-NH3 (pH 9.5)
B) methanol |
| Grandient: |
0-90% B (0-7 min) |
| Flow rate: |
0.2 ml/min |
| Temperature: |
25 °C |
| Detection: |
UV at 240 nm |
| Injection: |
1 μl |
| Column: |
YMC-TriArt C18 (3 μm, 12 nm) |
Flow rate: |
0.4 ml/min |
| Dimension: |
50 x 2.0 mm ID |
Temperature: |
25 °C |
| Eluent: |
A) acetonitrile / water / HCOOH (10/90/0.1)
B) acetonitrile / water / HCOOH (60/40/0.1) |
Detection: |
UV at 254 nm |
| Grandient: |
5-40% B (0-12 min) |
Injection: |
2 μl |
Application courtesy of YMC Co., Ltd.
Trademarks
Zorbax Eclipse Plus C18 RRHD C18 is a trademark of Agilent Technologies Inc.
Capcell Pak C18 AQ is a trademark of Shiseido Co., Ltd.
Inertsil and Inertsil ODS-3 are trademarks of GL Sciences Inc.
Acquity BEH C18, Atlantis T3, XBridge C18, XTerra MS C18 and SunFire C18 are trademarks of Waters Corp.
Gemini C18, Gemini NX C18 and Kinetex C18 are trademarks of Phenomenex, Inc.
Hypersil Gold is a trademark of Thermo Fisher Scientific Inc.
Eternity C18 is a trademark of EKA Chemicals AB.
Ascentis Express C18 is a trademark of Sigma-Aldrich Co.
TSKgel ODS-100S is a trademark of Tosoh Bioscience Inc.
The publication of the image and data material is courtesy of YMC Co., Lt
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