Comparison of separative selectivity

The selectivity characteristics of each column are shown using hydrophobicity and hydrogen-bonding ability as indicators. The ProFamily series of ODS phases is designed to make Hydrosphere C18 and Pro C18 RS have contrasting separation characteristics, with standard Pro C18 in between. Also, Pro C8 and C4 have different selectivity from the ODS phases. By choosing one from these 5 types of columns, one can easily optimise the separation of polar and non-polar compounds.