The base material for the stationary
phases of the ProFamily is silica, which is covered with silanol groups on the surface. In the derivatisation step, these groups are reacted with, for example, octadecylsilanes to give C18 reversed phases. To minimize unwanted interactions between residual silanols and the sample molecules that are responsible for asymmetric peaks, YMC utilises a proprietary, highly effective endcapping process.
In addition, silica with an extremely low metal content is used for the phases of the ProFamily to suppress polar interactions, as metal impurities influence silanol groups to become more acidic. The table above illustrates that the total metal content is lower than 10 ppm for those elements reported.
| Lot-to-lot reproducibility for Pro C18, 5 µm |
| In this comparison, phenol and pyridine were selected to show the peak performance for acidic and basic compounds, respectively. The low metal content and the proprietary endcapping result in a very good peak performance from lot-to-lot-to-lot. |
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