Basic compounds

Tricyclic antidepressants Column: YMC-Pack Pro C18 (5 μm, 12 nm) 150 x 4.6 mm i.d. Eluent: 20 mM potassium phosphate buffer (pH 6.9) / methanol (25/75) Flow rate: 1.0 ml/min Temperature: 37°C Detection: UV at 254 nm

YMC-Pack Pro C18 is based on an ultra pure silica support, which is used for the whole ProFamily. Due to a proprietary endcapping process especially designed for this type of silica, YMC-Pack Pro C18 is perfectly suitable for the separation of acidic and basic molecules. The inertness of the silica makes it an excellent choice for the analysis of drugs or metabolites, compounds that are susceptible to polar interactions with residual silanol groups and metal impurities as demonstrated in the following comparison. The extreme basic substances are selected to prove the very good performance of YMC-Pack Pro C18 in regard to their separation and the peak performance that cannot be achieved with classical materials.