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| Separation of extremely basic compounds |
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Tricyclic antidepressants
Column: 150 x 4.6 mm i.d.
Eluent: 20 mM potassium phosphate buffer (pH 6.9) / methanol (25/75)
Flow rate: 1.0 ml/min
Temperature: 37°C
Detection: UV at 254 nm |
| Separation of peptides under LC-MS compatible conditions |
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Tetracycline antibiotics
Column: YMC-Pack Pro C18 (5 μm, 12 nm, 150 x 4.6 mm)
Eluent: acetonitrile / 10mM oxalic acid (10/90)
Flow: 1.0 ml/min
Detection: UV at 280 nm, 0.13 AUFS
Temperature: 37 ºC
Injection: 6 μl (0.2 mg/ml) |
| Flavonoids |
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Antiepileptics
Column: YMC-Pack Pro C18 (5 μm, 12 nm, 150 x 4.6 mm)
Eluent: 50 mM NH4H2PO4-(NH4)2HPO4 (pH 6.7) / methanol (50/50)
Flow: 0.7 ml/min
Detection: UV at 215 nm, 0.32 AUFS
Temperature: 30 ºC
Injection: 8 μl (0.035 0.7 mg/ml) |
| Antiepileptics |
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Flavonoids
Column: YMC-Pack Pro C18 (5 μm, 12 nm, 150 x 4.6 mm)
Eluent: acetonitrile / 10 mM H3PO4 (35/65)
Flow: 1.0 ml/min
Detection: UV at 280 nm, 0.13 AUFS
Temperature: 37 ºC
Injection: 5 μl (0.05 mg/ml) |
| Separation of related cardiac glycosides |
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Cardiac glycosides
Column: YMC-Pack Pro C18 (5 μm, 12 nm, 75 x 4.6 mm)
Eluent: acetonitrile / water (10/90)
Flow: 1.0 ml/min
Detection: UV at 220 nm, 0.26 AUFS
Temperature: 37 ºC
Injection: 5 μl (0.2, 0.4 mg/ml) |
For more applications please refer to our “Application Data Collections” or contact us directly.
Column care
YMC Pack Pro C18 is stable towards hydrolysis between pH 2-8. Remove acid and buffer salts before storage. Store the
column in methanol / water = 70/30.
For detailed information please refer to the “Column Care and Use Instructions” which are shipped with each analytical column.
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