Problem: Conventional ODS under 100% aqueous chromatographic conditions*
Column: 150 x 4.6 mm i.d. Eluent: 20 mM KH2PO4-K2HPO2 (pH 6.9) Flow rate: 1.0 ml/min Temperature: 37 °C Detection: UV at 254 nm	After use, the column was stored overnight without any washing procedure. The next day, the application was continued.

Solution: Hydrosphere C18 under 100% aqueous chromatographic conditions*
Column: 150 x 4.6 mm i.d. Eluent: 20 mM KH2PO4-K2HPO2 (pH 6.9) Flow rate: 1.0 ml/min Temperature: 37 °C Detection: UV at 254 nm	After use, the column was stored overnight without any washing procedure. The next day, the application was continued.

*By courtesy of YMC Co., Ltd.

Column care
Hydrosphere C18 is stable towards hydrolysis between pH 2 - 8 in up to 100% aqueous systems and a maximum of 50 °C. Remove acid and buffer salts before storage. Store the column in methanol / water = 70/30.
For detailed information please refer to the “Column Care and Use Instructions” which are shipped with each analytical column.