Applications
The unique chemistry provides a phase with true reversed phase characteristics, high resolution and excellent peak symmetry for basic compounds without the need for ion pair reagents or amine modifiers (see separation of anilines using acetonitrile /water eluent). Unlike many base-deactivated phases, YMCbasic is also suitable for separation of acidic compounds, showing slight retention of highly polar acid compounds such as maleate. YMCbasic provides a complementing selectivity seen with conventional C8 and C18 materials, but without peak tailing for basic compounds.

Anilines

Column: YMCbasic, 4.6 x 250 mm Eluent: acetonitrile / water (60/40) Flow: 2.0 ml/min Detection: UV at 254 nm

Cough/cold medicine

Column: YMCbasic, 4.6 x 250 mm Eluent: acetonitrile / 0.05mM KH2PO4 (pH 3.2) (15/85) Flow: 2.0 ml/min Detection: UV at 254 nm

Basic compounds

Column: YMCbasic, 150 x 4.6 mm Eluent: 20mM K2HPO4-KH2PO4 (pH 6.9) / acetonitrile (92/8) Flow: 1.0 ml/min Detection: UV at 254 nm, 0.32 AUFS Temperature: 37 °C Pressure: 61 kgf/cm2 Injection: 10 μl (30 - 300 μg/ml)

YMCbasic is also available in preparative bulk quantities up to 50 μm particle size and larger (on request).

Column care
The recommended pH range for YMCbasic is 2-7,5. Remove acids and buffer salts before storage. Store the column in methanol/water=70/30. Clogged inlet frits often can be cleaned by changing the flow direction or replacement.
For detailed information please refer to the “Column Care and Use Instructions” which are shipped with each analytical column.