| Steroids |
|
Column:
Eluent:
Flow:
Detection:
Temperature:
Injection: |
YMC-Pack SIL (5 μm, 6 nm) 250 x 4.6 mm i.d.
hexane / THF / acetic acid (60/40/1)
1.5 ml/min
UV at 250 nm, 0.16 AUFS
30 °C
10 μl (0.25 mg/ml) |
| Tocopherols |
|
Column:
Eluent:
Flow:
Detection:
Temperature:
Injection: |
YMC-Pack SIL (5 μm, 12 nm) 150 x 4.6 mm i.d.
hexane / THF / acetic acid (97/3/0.25)
1.0 ml/min
UV at 295 nm, 0.08 AUFS
30 ºC
10 μl (0.20 ≈ 0.31 mg/ml) |
| Vitamin A fatty acid ester |
|
Column:
Eluent:
Flow:
Detection:
Temperature:
Injection: |
YMC-Pack SIL (5 μm, 12 nm) 150 x 4.6 mm i.d.
cyclohexane / dichloromethane / acetic acid (100/0.5/0.5)
1.0 ml/min
UV at 350 nm, 0.32 AUFS
30 ºC
5 μl (1.0 mg/ml) |
|
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