The selectivity of YMC-Pack Protein-RP is different from that seen with conventional wide
pore butyl phases and it is specifically suited for the protein analysis. In the applications
below it effectively separates both low molecular weight compounds and high molecular
weight proteins, with equally good peak shapes being obtained.
| Proteins |
|
Column:
Eluent:
Flow rate:
Temperature:
Detection: |
YMC-Pack Protein-RP (5 μm) 250 x 4.6 mm i.d.
A) water / TFA (100/0.1)
B) acetronitrile / TFA (100/0.1)
30%-90% B (0-45 min., linear)
1.0 ml/min
ambient
UV at 280 nm, 0.04 AUFS |
| Proteins and peptides |
|
Column:
Eluent:
Flow rate:
Temperature:
Detection: |
YMC-Pack Protein-RP (5 μm) 250 x 4.6 mm i.d.
A) water / TFA (100/0.1)
B) acetronitrile / TFA (100/0.1)
10%-90% B (0-60 min., linear)
1.0 ml/min
ambient
UV at 220 nm, 0.32 AUFS |
|
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