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Analytical Columns

 

YMC-Pack Polyamine II
 


Mono- and Disaccharides
Mono- and Disaccharides1: Fructose
2: Glucose
3: Sucrose
4: Maltose
5: Lactose
Column:
Eluent:
Flow:
Detection:
Temperature:
YMC-Pack Polyamine II (250 x 4.6 mm)
ACN/H2O = 75/25
1.0 ml/min
RI, 32x10-6 RIU/FS
26 ºC

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Pyridylamino (PA)-Sugar Chains
G: Galactose
GN: N-Acetylglucosamine
M: Mannose
F: Fructose
PA: Pyridylamino
Column:
Eluent:
Flow:
Detection:
Temperature:
YMC-Pack Polyamine II (150 x 4.6 mm)
Methanol/NH4H2PO4 (20 mM) = 80/20
1.0 ml/min
FLS 320 nm, Em 400 nm, 4 mV/FS
37 ºC

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NP-Separation of Tocopherols
NP-Separation of Tocopherols1: α-Tocopherol
2: β-Tocopherol
3: γ-Tocopherol
4: δ-Tocopherol
Column:
Eluent:
Flow:
Detection:
Temperature:
YMC-Pack Polyamine II (250 x 4.6 mm)
Hexane/Ethyl acetate = 70/30
1.0 ml/min
UV, 295 nm
30 ºC

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Maltooligosaccharides
Maltooligosaccharides1: Maltoundecaose (G11)
2: Maltododecaose (G12)
3: Maltotridecaose (G13)
4: Maltotetradecaose (G14)
5: Maltopentadecaose (G15)
Column:
Eluent:
Flow:
Detection:
Temperature:
YMC-Pack Polyamine II (250 x 4.6 mm)
ACN/H2O = 55/45
1.0 ml/min
RI, 32 x 10-6 RIU/FS
26 ºC

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