YMC-Pack Phenyl provides a unique selectivity when compared to aliphatic straight chain reversed phases such as C18, C8 or C4. The p-electrons of the phenyl groups can interact with aromatic residues of an analyte molecule in addition to hydrophobic interactions to increase retention relative to non-aromatic species.
Phenyl phases are convenient for the separation of aromatic compounds and also provide a useful alternative to C18 or C4 phases for the separation of peptides and proteins on both small pore (12 nm) and wide pore (30 nm) materials. Retention is decreased on wide pore phenyl phases relative to 12 nm phenyl material due to the lower surface area of the wide pore material.

Column care
YMC-Pack Phenyl is stable towards hydrolysis between pH 2-7,5. Remove acid and buffer salts before storage. Store the column in methanol / water = 70/30. Clogged inlet frits often can be cleaned by changing the flow direction or replacement.
For detailed information please refer to the “Column Care and Use Instructions”, which are shipped with each analytical column.