Applications
YMC-Pack ODS-AQ is a C18 reversed phase silica based HPLC packing material specifically designed for use in 100% aqueous eluents. As a result of the proprietary derivatisation process, YMC-Pack ODSAQ exhibits a different selectivity to that of traditional C18 stationary phases. This difference in selectivity of YMC-Pack ODS-AQ can be used to advantage for HPLC separations, which are difficult to achieve with conventional C18 columns.
| Exceptional performance for the separation of polar compounds |
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| Cephalosporin antibiotics |
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Cephalosporin antibiotics |
| Column: |
YMC-Pack ODS-AM (12 nm, 5 μm, 150 x 4.6 mm) |
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Column: |
YMC-Pack ODS-AQ (12 nm, 5 μm, 150 x 4.6 mm) |
| Eluent: |
methanol/water/acetic acid (10/85/5) |
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Eluent: |
methanol/water/acetic acid (10/85/5) |
| Flow: |
1.0 ml/min |
|
Flow: |
1.0 ml/min |
| Detection: |
UV at 260 nm, 0.16 AUFS |
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Detection: |
UV at 260 nm, 0.16 AUFS |
Temperature: |
37 °C |
|
Temperature: |
37 °C |
| Strong retention in aqueous eluents |
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| Crude drugs |
| Column: |
YMC-Pack ODS-AQ (12 nm, 5 μm, 250 x 4.6 mm) |
| Eluent: |
20mM H3PO4 - NaH2PO4 (pH 2.8) |
| Gradient: |
0-100 % B (0-20 min, linear), 100 % B (20-40 min) |
| Flow: |
0.7 ml/min |
| Detection: |
UV at 220 nm, 0.08 AUFS |
| Temperature: |
30 °C |
Comparison of YMC-Pack ODS-AQ with competitive products
Since 1987, YMC-Pack ODS-AQ has consistently increased its popularity due to its novel selectivity pattern towards polar compounds and its ability to withstand 100% aqueous conditions. Today, more than 19 (!) years later, many new analytical and preparative methods are still being developed on YMC-Pack ODS-AQ chemistry in spite of various AQ-type products recently introduced by our competitors; phases with supposedly “identical” selectivity or with exotic bonding techniques designed to generate performance characteristics similar to those of YMC-Pack ODS-AQ. However, genuine YMC-Pack ODS-AQ still represents today a fully competitive state-of-the-art high performance stationary phase, despite the complementary YMC innovation, namely new generation Hydrosphere C18, as a potential in-house competitor.
| Tryptic digest of BSA |
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