| 16 PAH according to EPA 610* |
 |
| Column: |
YMC PAH (3 μm) 50 x 4.0 mm i.d. |
|
Gradient |
|
|
| Eluent: |
A) methanol / water (80/20) |
|
Time [min] |
A |
B |
| |
B) acetonitrile |
|
0.0 |
90% |
10% |
| Flow: |
2.0 ml/min |
|
0.5 |
90% |
10% |
| Detection: |
UV at 254 nm |
|
1.0 |
0% |
100% |
Temperature: |
24 °C |
|
4.0 |
0% |
100% |
| 16 PAH according to EPA 601* |
 |
| Column: |
YMC PAH (5 μm) 250 x 3.0 mm i.d. |
| Eluent: |
A) MeOH / water (80/20) |
| |
B) acetonitrile |
| Flow: |
0.43 ml/min |
| Detection: |
UV at 254 nm |
| Temperature: |
30 °C |
| PCB separation according EPA* |
 |
| Column: |
YMC PAH (3 μm) 100 x 3.0 mm i.d. |
| Eluent: |
CH3CN / water (75/25) (v/v) |
| Flow: |
0.6 ml/min |
| Detection: |
UV at 220 nm |
| Temperature: |
30 °C |
Polynuclear Aromatic Hydrocarbons (PAHs) are ubiquitous xenobiotics which are known or suspected carcinogens. According to the German Trinkwasserverordnung (TVO) six PAH have to be quantified. Moreover Perylene, which is often present in the samples under investigation, has to be fully resolved in order to avoid coelutions and therefore questionable results.
The chromatogram below shows the successful separation of all seven substances with a YMC PAH column as stationary and an acetonitrile/methanol mixture as a simple isocratic mobile phase. The elution time has been reduced to approximately six minutes with excellent resolution without the need for gradient elution.
| 7 PAH according to EPA 610* |
| |
| Column: |
YMC PAH (5 μm) 125 x 4.0 mm i.d. |
|
1. Fluoranthene |
| Eluent: |
acetonitrile / methanol (95/5) |
|
2. Benzo[b]fluoranthene |
| Flow: |
1.0 ml/min |
|
3. Perylene |
| Detection: |
UV at 254 nm |
|
4. Benzo[k]fluoranthene |
| Temperature |
25 °C |
|
5. Benzo[a]pyrene |
| Inj.-vol.: |
5 μm |
|
6. Benzo[ghi]perylene |
| |
|
|
7. Indeno[1,2,3-cd]pyrene |
*Source: Courtesy of YMC Europe GmbH |
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