High binding capacity and high recovery
The porous type of YMC-BioPro shows great absorption capacity and excellent recovery, and is especially suitable for semipreparative
separations of proteins and antibodies.
Comparison of dynamic binding capacity (DBC) for BSA
Compared with conventional porous
polymer anion-exchange
columns, YMC-BioPro QA gives
higher DBC and recovery rates.
This indicates that YMC-BioPro
has a much lower nonspecific
adsorption compared to conventional
columns.
| |
Dynamic binding capacity
(mg/ml-gel, 10% breakthrough) |
Eluted amount
(mg/ml-gel) |
Recovery*
(%) |
| YMC-BioPro QA |
126 |
120 |
95 |
| Mono Q |
100 |
35 |
35 |
| BioAssist Q |
73 |
58 |
79 |
|
| *Recovery: (Eluted amount/Dynamic binding capacity) x 100 |
High Mass Recovery
The high purity silica support and complete bonding coverage of Protein-RP results in very high sample recoveries. This is an important consideration when choosing a column for biochemical analyses. The table below shows recoveries of Proteins and Peptides. YMC-Pack Protein-RP provides higher recoveries than competitive C4 columns.
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