Standard protein separation on YMC-BioPro SP*
Eluent:   A) 20 mM KH2PO4-K2HPO4 (pH 6.8) B) 20 mM KH2PO4-K2HPO4 (pH 6.8) containing 0.5M NaCl 0-100% B (0-60 min., linear) Flow rate:   0.5 ml/min (4.6 mm i.d. column) 0.59 ml/min (5.0 mm i.d. column) Temperature:   25 °C Detection:   UV at 220 nm Injection:   20 μl (4.6 mm i.d. column) 23.6 μl (5.0 mm i.d. column)	1.   Cytochrome c (0.5 mg/ml) 2.   Ribonuclease A (0.5 mg/ml) 3.   Lysozyme (0.5 mg/ml)

*By courtesy of YMC Co., Ltd.