Due to the production processes used to manufacture YMC-Pack ProFamily, methods can be easily downscaled with unchanged selectivity. As the examples shown demonstrate, conventional HPLC methods can be transferred easily to Ultra Fast LC methods by choosing YMC-UltraHT columns to gain effi ciency and signifi cantly reduce analysis time.
How does it work in practice?
The example below shows how easy it is to down-scale an existing method. The compounds of interest are flavonoids.
The conventional HPLC is carried out on 150 x 4.6 mm ID packed with 5 μm. The last eluting peak appears after 25 minutes.
| Conventional LC method |
|
Column:
Part No.:
Flow rate:
Injection:
Eluent:
Temperature:
Detection:
Sample: |
YMC-Pack Pro C18 (5 μm, 12 nm) 150 x 4.6 mm ID
AS12S051546WT
1.0 ml/min
5 μl
acetonitrile/water/formic acid (35/65/0.1)
40 °C
UV at 260 nm
0.05 mg/ml |
| Ultra-fast LC method |
|
Column:
Part No.:
Flow rate:
Injection:
Eluent:
Temperature:
Detection: |
YMC UltraHT Pro C18 (2 μm, 12 nm) 50 x 2.0 mm ID
AS12S020502WT
0.2 ml/min (1.06 mm/sec)
1 μl
acetonitrile/water/formic acid (35/65/0.1)
40 °C
UV at 260 nm |
| Ultra-fast LC method with increased flow |
|
Column:
Part No.:
Flow rate:
Injection:
Eluent:
Temperature:
Detection: |
YMC UltraHT Pro C18 (2 μm, 12 nm) 50 x 2.0 mm ID
AS12S020502WT
0.6 ml/min (3.18 mm/sec)
1 μl
acetonitrile/water/formic acid (35/65/0.1)
40 °C
UV at 260 nm |
|
Start
