|
|
 |
|
|
|
| For separation of proteins with molecular weights from 10.000 to several 100.000 |
 |
| Column: |
YMC-Pack Diol, 300 x 8.0 mm ID |
| Eluent: |
0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl |
| Flow rate: |
0.5 ml/min |
| Detection: |
UV at 280 nm |
Temperature: |
25 °C |
| Plasma constituents |
 |
| Column: |
YMC-Pack Diol-300 + Diol-200, 300 x 8.0 mm ID x 2 |
|
Temperature: |
ambient (25 °C) |
| Ordering-No.: |
DL30S053008WT + DL20S053008WT |
|
Detection: |
UV at 280 nm |
| Eluent: |
0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl |
|
Injection: |
20 μl (L: 1 μl) |
| Flow rate: |
0.5 ml/min |
|
Sample |
A) 100 μl/ml; B-M) 1.0 mg/ml |
| Plasmid pBR322 restriction and pBR322 Hae III restriction fragment |
 |
| Column: |
YMC-Pack Diol-300 +
Diol-200, 500 x 8.0mm ID x 2 |
|
Column: |
YMC-BioPro QA-F (5 μm), 100 x 4.6 mm ID |
| Ordering-No.: |
DL30S055008WT + DL20S055008WT |
|
Ordering-No.: |
QF00S051046WT |
| Eluent: |
0.1 M KH2PO4-K2HPO4 (ph 7.0) containing 0.2M NaCl |
|
Eluent: |
A) 20 mM Tris-HCI (pH 8.1) |
| B) 20 mM Tris-HCI (pH 8.1) containing 0.1 M NaCl 70-85% B (0-20 min), 85% B (20-25 min) |
| Flow rate: |
0.7 ml/min |
|
Flow rate: |
0.5 ml/min |
| Temperature: |
ambient (25 °C) |
|
Temperature: |
35 °C |
| Detection: |
UV at 260 nm |
|
Detection: |
UV at 260 nm |
| Injection: |
10 μl |
|
Injection: |
10 μl |
| Proteins in human serum |
 |
| Eluent: |
0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl |
| Flow rate: |
0.5 ml/min |
| Temperature: |
ambient (25 °C) |
| Detection: |
UV at 280 nm |
| Injection |
20 μl |
| Sample.: |
Human serum (100 μl/ml) |
| Monoclonal antibody (MAb) |
 |
| Column: |
YMC-Pack Diol-200, 300 x 4.6 mm ID |
| Ordering-No.: |
DL20S053046WT |
| Eluent: |
0.1 M KH2PO4-K2PO4 (pH 7.0) |
| Flow rate: |
0.17 ml/min |
| Temperature: |
ambient (25 °C) |
| Detection: |
UV at 220 nm |
| Injection: |
10 μl |
| Sample.: |
a commercially available mouse monoclonal lgG1 (0.05 mg/ml) (purified by DEAE chromatography, containing NaN3) |
| Proteins for molecular weight marker |
 |
| Column: |
YMC-Pack Diol-200, 300 x 8.0 mm ID |
| Ordering-No.: |
DL20S053008WT |
| Eluent: |
0.1 M KH2PO4-K2PO4 (pH 7.0) containing 0.2 M NaCl |
| Flow rate: |
0.7 ml/min |
| Temperature: |
ambient (26 °C) |
| Detection: |
UV at 280 nm, 0.08 AUFS |
| Injection: |
15 μl (100 μl / 1 vial) |
| Sample.: |
MW-Marker (HPLC), manufactured by ORIENTAL YEAST CO., LTD. |
|
|
|
| Column: |
YMC-Pack Diol-300, 500 x 8.0 mm ID |
|
Column: |
YMC-Pack Diol-300, 500 x 8.0 mm ID |
| Ordering-No.: |
DL30S055008WT |
|
Ordering-No.: |
DL30S055008WT |
| Eluent: |
water |
|
Eluent: |
water |
| Flow rate: |
1.0 ml/min |
|
Flow rate: |
1.0 ml/min |
| Temperature: |
ambient (24 °C) |
|
Temperature: |
ambient (24 °C) |
| Detection: |
RI, 32 x 10-6 RIU/FS |
|
Detection: |
RI, 32 x 10-6 RIU/FS |
| Injection: |
20 μl (5.0 mg/ml) |
|
Injection: |
20 μl (5.0 mg/ml) |
| Keratan polysulfat |
Cyclodextrins |
|
|
|
| Column: |
YMC-Pack Diol-300, 500 x 8.0 mm ID |
|
Column: |
YMC-Pack Diol 60, 500 x 8.0 mm ID |
| Ordering-No.: |
DL30S055008WT |
|
Ordering-No.: |
DL06S055008WT |
| Eluent: |
water |
|
Eluent: |
water |
| Flow rate: |
1.0 ml/min |
|
Flow rate: |
1.0 ml/min |
| Temperature: |
ambient (24 °C) |
|
Temperature: |
ambient (24 °C) |
| Detection: |
RI, 32 x 10-6 RIU/FS |
|
Detection: |
RI, 32 x 10-6 RIU/FS |
| Injection: |
20 μl (5.0 mg/ml) |
|
Injection: |
30 μl (1.67 mg/ml) |
|
|
 |
Start
