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YMC HILIC Columns for Polar Analytes

Hydrophilic Interaction Chromatography (HILIC) Applications Literature

• Nucloesides and bases
• Acrylamide
• Melamine in powdered cheese
• Melamine in dairy products: HILIC versus RP
• Reproducibility of YMC HILIC
• Comparison of YMC HILIC Diol with other conventional HILIC phases
• Comparison of YMC HILIC Diol with other conventional HILIC phases

Nucloesides and bases
YMC-Pack Diol-NP 5 μm, 2.0 x 150 mm ZIC-HILIC 5 μm, 2.1 x 150 mm XBridge HILIC 5 μm, 2.1 x 150 mm LUNA HILIC 5 μm, 3.0 x 150 mm
1. Uracil		Eluent:	water / acetonitrile (10/90) containing 10mM CH3COONH4
2. Uridine		Flow rate:	0.2 ml/min (Diol-NP), 0.22 ml/min (ZIC-HILIC, XBridge), 0.45 ml/min (LUNA)
3. Adenosine		Temperature:	30 °C
4. Cytosine		Detection:	UV at 254 nm
5. Cytidine
6. Guanosine

Acrylamide
Since the detection of the genotoxic compound, acrylamide in starchy foodstuffs subjected to prolonged heating, the analysis of this compound has been of major interest.
Column:	YMC-Pack Diol-NP (5 μm, 12 nm) 150 x 2.0 mm ID
Eluent:	20 mM CH3COONH4 / acetonitrile (5/95)
Flow rate:	0.2 ml/min
Temperature	25 °C
Detection:	UV at 210 nm
Injection:	1 μl (40 μg/ml)

Melamine in powdered cheese
Column:	YMC-Pack Diol-NP (5 μm, 12 nm) 150 x 3.0 mm ID.
Eluent:	10 mM CH3COON4 / methanol (5/95)
Flow:	0.4 ml/min
Detection:	UV at 210 nm
Injection:	5 μl
Temperature:	40 °C

Melamine in dairy products: HILIC versus RP
A) Powdered cheese (blank B) Melamine-spiked powdered cheese (10 mg/kg; final 0.5 μg/ml) C) Standard (0.5 μg/ml) YMC-Pack Diol-NP (5 μm, 12 nm) 150 x 2.0 mm ID YMC-UltraHT Hydrosphere C18 (2 μm, 12 nm) 50 x 2.0 mm ID
	Eluent:	10 mM CH3COONH4 / methanol (5/95)		Eluent:	A)	water / heptafluorobutyric acid (100/0.1)
					B)	methanol / heptafluorobutyric acid (100/0.1) 0-3 min (0-90% B)
	Flow rate:	0.2 ml/min		Flow rate:	0.4 ml/min
	Temperature	40 °C		Temperature	40 °C
	Detection	UV at 210 nm		Detection:	UV at 210 nm

Reproducibility of YMC HILIC
Column:	YMC-Pack Diol-NP (5 μm, 12 nm) 150 x 2.0 mm ID.
Eluent:	10 mM CH3COON4 / methanol (5/95)
Flow:	0.2 ml/min
Detection:	UV at 210 nm
Temperature:	40 °C
Sample:	melamine-spiked powdered cheese 0.5 μg/ml)