While different column hardware manufacturers have introduced a variety of connecting port geometries throughout the years, the Sure-Fit™-Connector represents an ideal tool to avoid dead volumes, irrespective of the column brand, when connecting different columns to a given HPLC system.
YMC highly recommends the use of the Sure-Fit™-Connector due to its internal spring tensioned mechanism that automatically self-adjusts to any port depth while maintaining constant pressure on the capillary tubing to guarantee a connecting free from any dead volume. Thus, column changeovers are easy, especially because there are no tools required. The Sure-Fit™-Connector is fingertight to 6000 psi and is available in a range of capillary tubing i.d.’s and lengths.
Click here to download the SureFit Flyer for more information.
Analytical guard cartridge systems
YMC offers two different analytical guard cartridge holder concepts. A universal finger tight system (XPGCH-Q1 for 10 mm guard and XPGCH-Q2 for 20 mm guard and all internal diameters for 1.0 to 4.6mm id columns). This system guarantees a low dead volume connection between the guard cartridge and the main column irrespective of the type of column fitting used. To connect the guard cartridge holder to the column to give leak-proof connection up to 6000 psi follow the description below.
Semiprep guard cartridge systems
For semi-prep columns cartridges and holders of 10 mm length and 8/20 mm inner diameter are available (more dimensions on request). The 8 mm guard cartridge corresponds to the 10 mm i.d. semi-prep column and the 20 mm guard cartridge to the 20 mm i.d. semi-prep column, respectively. As the holder for these cartridges feature Valco-compatible fittings (pilot depth 0.08 inch) it is essential that slip-free fittings are used or specially made stainless steel fittings and stainless steel capillaries are made up so that differences between Valco-compatible, Parker-compatible (pilot depth 0.09 inch) and/or Waters-compatible (pilot depth 0.13 inch) column endfittings can be accommodated.
Due to the higher flowrates involved
in semi-prep and prep chromatography the
use of PEEK capillaries should be avoided
Preparative guard cartridge systems
Guard cartridges for preparative-scale columns should be of the same internal diameter as the column and either 50 or 100mm in length. Connections should be either 1/16 or 1/8 inch stainless steel capillaries, depending on flowrate and column size with the appropriate stainless steel nuts and ferrules.