HPLC 2009 - HPLC Symposium in Dresden

We are pleased to announce that YMC Europe GmbH is again attending this year’s HPLC 2009, which will be held in Dresden. We are delighted to have the possibility of welcoming our guests to one of Germany’s most attractive cities.

The HPLC Symposium Series represents the premier conference for liquid phase separations and related techniques. In previous years, it has always been a great event for all interested parties, from scientist to CEOs and from customers to manufacturers.

Traditionally, these HPLC Symposia present new developments in the field, a meeting point and discussion forum for scientists and users of high-performance liquid chromatography.

YMC will take this opportunity to show the following posters on the poster-session:

YMC-BioPro - (LSC47-We)
A Newly Developed Hydrophilic Polymer-Based Ion Exchange
Chromatography Column for Separation of Various Biological Molecules

YMC-UltraHT Pro C18 RS 2µm - (FBA54-Tu)
High-throughput HPLC Analysis of Anthocyanins and Anthocyanidins
in Bilberry Using a Novel 2 micron Polymeric-C18 Column with
High Chemical Stability and Unique Selectivity

YMC Exphere - (SPI40-Th)
Newly Developed High Strength and Chemically Stable Silica Gel
Based Preparative Reversed Phase Packing Materials

For YMC, as a manufacturer, this is one of the most important opportunities for us to get together with our clients and to hear the latest inventions, trends and especially the requirements which are expected to be fulfilled by a leading manufacturer of high quality silica based materials such as ourselves.

We would be glad welcoming you on our booth, which you will find in the vendor exhibition area on stand number 58/59.

Opening hours of the exhibition are as follows:

If you are unable to attend the complete conference, we recommend planning your visit to the exhibition for Thursday, July 2nd, as the organisers allows visiting the exhibition free of charge for this day only. For all other dates there will be a 75€ charge for visitors.

If you require more detailed information or have any questions please do not hesitate to contact Mr. Marc Ujma.

Simultaneous separation of pesticides by LC/MS

With the constantly rising population and frequent uncertain weather, the need for high yielding agriculture is paramount. This means that use of plant protection agents, such as herbicides and insecticides is necessary, in order to secure harvests. However, in addition to the positive effects of such agents, severe side effects can be caused if high-level doses are ingested. As a result the use of these substances is strictly controlled, in order to protect the consumer’s health. Nevertheless, recent investigations have shown that restrictions have been violated in the past and sensitive fruit such as strawberries and grapes are frequently in the news for being contaminated.
There are currently about 1300 different active substances in use and only about half of them can be detected. However, they can cause minor irritations, temporary allergic reactions or even substantial damage to the immune system. The number of consumers who exhibit allergic reactions is also rising. Threshold Limit values for pesticides are specified by the European Union commission. However not all pesticides are covered and, in order to fill these gaps, highly efficient, fast analysis methods are required. Since the sample composition can be very complex, it is necessary to develop simple, generic sample preparation methods linked to fast analysis method for pesticides in food. Especially suitable for this purpose are HPLC methods or ultra-fast LC applications.

The analysis of structurally very similar polar substances is frequently unsuccessful, due to the insufficient selectivity of many conventional C18 phases. AS a result, YMC-UltraHT ProC18 was selected as the stationary phase. It is characterised by a balanced phase modification, which allows analysis of both very polar and moderately polar structures successfully.

Figure 1

Figure 2

Method development
The HPLC methods have been carried out on a Jasco X-LC system at ambient temperature with ESI-MS detection. It was found that using acetonitrile/water gradient eluents containing ammonium acetate gave good separation of all components (fi g. 1).
The chromatographic analysis of a mixture consisting of 26 pesticides showed a very good separation when using a YMC-Pack Pro C18 3 μm, 150 x 2.0 mm ID column. The chromatographic results are obtained within 21 minutes.
In figure 2 the method transfer onto a 75 x 2.0 mm ID YMC-UltraHT 2 μm column is shown. The flow rate has been increased from 0.2 ml/min to 0.4 ml/min in order to maintain the gradient profile. The retention pattern has not been altered, but the retention has been decreased to 9 minutes.

Results and discussion
The strict monitoring of pesticides used ever increasingly in agriculture increases the demand for sensitive and fast analytical LC methods.
Therefore the need for downscaling of existing methods is one of the challenges that chromatographers meet. The method developed in this study shows the simple method transfer from a conventional HPLC method developed on YMC-Pack Pro C18 3 μm to an ultra-fast LC application. The use of the new YMC UltraHT ProC18, with its particle size of 2 μm enables downscaling without any further method development. The combination of small particles and short column lengths results in shorter retention times without compromising resolution.

Application Courtesy of J. Watanabe, TAKARA BIO INC.

YMC HILIC Columns for Polar Analytes

In general, reliable applications in HPLC require a minimum retention factor (k’) of 2. In the widely used reversed phase methods polar compounds such as peptides, organic acids or pyrimidines often exhibit little or no retention and therefore low resolution on a conventional C18 phase. The use of additives such as ionpair reagents is not an alternative since it is not applicable to LC-MS. Also normal phase chromatography fails due to poor solubility of the polar compounds in organic solvents. HILIC fills the gap between reversed phase and normal phase chromatography, because polar compounds can be retained while using e.g. acetonitrile/water eluents and buffers suitable for LC-MS.

Order your copy now or click here to download it.

For further information, please feel free to contact
Dr. Friederike Becker.

Recertification according to ISO 9001:2008

YMC Europe GmbH is recertificated by Moody International according to DIN EN ISO 9001:2008 on 08.04.2009. Our current certificate is valid up to May 2012. You can find it <<<HERE>>>.