YMC HILIC Columns for Polar Analytes

In general, reliable applications in HPLC require a minimum retention factor (k’) of 2. In the widely used reversed phase methods polar compounds such as peptides, organic acids or pyrimidines often exhibit little or no retention and therefore low resolution on a conventional C18 phase. The use of additives such as ionpair reagents is not an alternative since it is not applicable to LC-MS. Also normal phase chromatography fails due to poor solubility of the polar compounds in organic solvents. HILIC fills the gap between reversed phase and normal phase chromatography, because polar compounds can be retained while using e.g. acetonitrile/water eluents and buffers suitable for LC-MS.

Order your copy now or click here to download it.

For further information please feel free to contact
Dr. Friederike Becker.

Discover the benefits of the new YMC*Gel HG-silica-base

NEW YMC*Gel HG-Type silica phases offer added value at no extra cost.

The continuous improvement of our bulk material is a central issue at YMC. Combining a new base silica with narrower particle- and pore-size distribution gives rise to our new YMC*Gel HG-phases.

The benefits for your applications include:

• Even greater reproducibility
• Lower operating pressures
• Increased durability, longer life cycle
• Further improvement in packing performance

Simply by looking at the new particles under the microscope, the differences to conventional base silica material become apparent. Scanning electron microscope (SEM) images clearly show that the particle surfaces of the HG-Phases are perfectly round and smooth. Furthermore, the HG-Phase material shows virtually no hollow “balloon-particles” under the light microscope. (see images below)

Trusted selectivity - always available:

YMC’s customers have put their trust in us for more than 30 years for one important reason: our unchanged product availability. This is why all those materials you currently use will continue to be available. Any phases which are used or will be used in validated processes or methods will continue to be available for the entire life cycle of your products.

In order to meet our customers demands regarding cost and process efficiency we will be offering the YMC*Gel HG-Phases for all new inquiries and projects.

The following YMC*Gel HG-Phases are now available:

The HG-Phases are available in particle sizes from 10μm to 50 μm and pore sizes from 12nm to 30nm in multi-ton quantities if required. Please contact us for a list of all available phases.

If you need more information, or if you want to request a sample, please contact: