Common problems during HPLC operations include peak shape anomalies such as peak tailing and split peaks. In order to remove these problems, the cause must be precisely determined. The majority of cases are the result of inappropriate conditions of separation; including inappropriate selection of a column or solvent, or use of an old column which has a void in the packing at the top of the packing. Here we discuss the method of determining the cause of the problem with peak shapes.
The simplest way is to test the column performance according to "shipping inspection criteria" in the column inspection report which is included with every column. If the examination reveals no peak shape anomalies, the cause will be the result of inappropriate selection of separation conditions. The separation conditions, including eluent selection, will have to be revised.
However, if the initial column test reveals any anomaly, the column may be faulty. Washing the column to remove an accumulated impurity or replacement of the column is necessary. We recommend examining column performances on a regular basis and always under the identical conditions.
YMC provide analytical criteria, including concentrations for test components, in column inspection reports to allow customers test the column performance of themselves. |
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